Nanopore Movies

Typical ion current recordings using the alpha-hemolysin nanopore.
The recording at the top shows the current in the open channel absent DNA or RNA. This open channel current can remain stable for many hours. The recording at the bottom shows the current in the same channel in the presence of poly C RNA 100mers. Each downward deflection indicates current impedance as a single RNA molecule transits the pore. These experiments were performed in 1M KCl at 23 °C and 120 mV applied potential using a 10 kHz low-pass filter. The poly C concentration was about 1 x 10^-6 M.

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